Separation of Glutathione Reductase in Human Serum by Gradient Gel Electrophoresis

Gradient gel electrophoresis of human plasma lipoproteins.

A rapid and simple method for separating and identifying plasma lipoproteins is described. The technique utilizes the “molecular sieving” properties of continuous concave gradient electrophoresis on a slab of polyacrylamide (4 to 26%) gel. Gradient gel electrophoresis is particularly suitable for separating lipoproteins according to their molecular weight. The technique can be used to demonstra...

Behavior of the Serum proteins in starch-agar-gel electrophoresis

Characterization of wine yeasts by temperature gradient gel electrophoresis (TGGE).

18S rDNA from 74 wine yeast strains was amplified by PCR using specific primers, and the products analyzed by temperature gradient gel electrophoresis (TGGE). TGGE is a useful method in screening the genotypes of the wine yeasts. Intraspecific differentiation was achieved on the basis of TGGE in some cases, whereas in others identical bands for strains classified as separate species were obtain...

Separation of low molecular weight serum proteins using acetonitrile precipitation assessed by one dimensional gel electrophoresis

In human serum, low molecular weight (LMW) proteins play an important role in major biological processes which were masked by high molecular weight (HMW) proteins. Separations and identification of LMW proteins of interest from serum is very intricate task. The objective of the present study was to develop in-house acetonitrile (ACN) precipitation method for separation of LMW proteins from high...

Fractionation of heparin-derived oligosaccharides by gradient polyacrylamide-gel electrophoresis.

Heparin-derived oligosaccharides, prepared by using flavobacterial heparinase, having a high degree of heterogeneity (sequence variability) were resolved into sharp well-defined bands by using polyacrylamide gel electrophoresis (PAGE). The use of a stacking gel and a high-density-pore-gradient resolving gel was primarily responsible for the success of this separation. Low-Mr standards of known ...